Immunoblot Analysis of Cell Death Regulators

Immunoblot was performed as described previously (Zhang et al., 2021b (link)). Antibodies used included: mouse caspase-1 (clone 4B4) [a kind gift from V. M. Dixit (Genentech, USA)], mouse GSDMD (ab209845, Abcam, UK), pro-caspase-8 (4790, Cell Signaling Technology, USA), mouse cleaved-caspase-8 (9429, Cell Signaling Technology), mouse RIP3 (homemade), mouse MLKL (homemade), human caspase-8 (ALX-804-242-C100, Enzo Life Sciences, USA), caspase-3 (9662, Cell Signaling Technology), human RIP3 (13526, Cell Signaling Technology), p38α (9228, Cell Signaling Technology), p-p38 (9211, Cell Signaling Technology), ERK (9107, Cell Signaling Technology), p-ERK (4377, Cell Signaling Technology), JNK (9252, Cell Signaling Technology), p-JNK (9251, Cell Signaling Technology), mouse p38α (homemade), Tubulin (M20005M, Abmart, China), Actin (homemade), and GAPDH (AC002, ABclonal, China).

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Gao X., Teng T., Liu Y., Ai T., Zhao R., Fu Y., Zhang P., Han J, & Zhang Y. (2023). Anthrax lethal toxin and tumor necrosis factor-α synergize on intestinal epithelia to induce mouse death. Protein & Cell, 15(2), 135-148.

Publication 2023

mouse caspase-1 (clone 4B4) pro-caspase-8 caspase-3 p-ERK p-JNK GAPDH

Corresponding Organization : Xiamen University

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of caspase-1, GSDMD, pro-caspase-8, cleaved-caspase-8, RIP3, MLKL, caspase-3, p38α, p-p38, ERK, p-ERK, JNK, p-JNK

control variables

Tubulin
Actin
GAPDH

controls

Positive controls: None mentioned
Negative controls: None mentioned

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