Immunofluorescence staining was carried out as described previously (24 (link), 27 , 28 (link)). Tissue slides were incubated at 4°C with primary anti-F4/80 (Bio-rad, F4/80_MCA497, 1:100), anti-CD206 (Proteintech, CD206_60143–1-Ig, 1:100), and anti-collagen alpha-1(I) (Col-1) (SouthernBiotech_Col-1_1310–01, 1:100) antibodies. Next slides were washed with PBS and then incubated at room temperature with Alexa Fluor® 488 AffiniPure Donkey Anti-Rat IgG (Jackson ImmunoResearch, 712–545-150, 1:100), Alexa Fluor® 594 Donkey Anti-Mouse IgG (abcam, ab150108, 1:100), or DyLight™ 405 AffiniPure Donkey Anti-Goat IgG (Jackson ImmunoResearch, 705–475-003, 1:50) for 1 h followed by washing with PBS and mounting. Stained slide samples were analyzed using a fluorescence microscope (Olympus IX71, Olympus Life Science, Japan). For statistical analysis by ImageJ, five areas (200 μm X 200 μm) were randomly selected from each sample and quantified.