Migratory ability was assessed by a wound healing assay as described previously (Zhao et al, 2012 (link)). Cells were seeded into a 6-well plate and allowed to grow to 90–100% confluence in DMEM, and the cell monolayers were then wounded by a 200 μl pipette tip. The wounded monolayers were washed four times with phosphate-buffered saline and incubated in serum-deprived DMEM with or without dimethyl-α-ketoglutarate (α-KG; Sigma Aldrich) for the indicated times (Csibi et al, 2013 (link)). Distances covered by migrated cells were quantified.
Protocol detail
Wound Healing Assay for Cell Migration
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Other organizations : Osaka University
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Variable analysis
independent variables
- Presence or absence of dimethyl-α-ketoglutarate (α-KG)
- Distances covered by migrated cells
- Cell seeding density (90-100% confluence)
- Cell culture medium (DMEM)
- Wound creation (using a 200 μl pipette tip)
- Washing of wounded monolayers (4 times with phosphate-buffered saline)
- Incubation time (not explicitly mentioned)
- Positive control: Not specified
- Negative control: Cells incubated in serum-deprived DMEM without dimethyl-α-ketoglutarate (α-KG)
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