Determining Antimicrobial Susceptibility Profiles

MICs of antibiotics and disinfectants were determined by microdilution in LB broth for antibiotics or Mueller-Hinton broth (BD Biosciences) for disinfectants, as described previously (35 (link)). Antibiotic agents, such as colistin, meropenem, amikacin, and ciprofloxacin, and disinfectants, such as ethanol, H₂O₂, SDS, and benzalkonium, were purchased from Fujifilm Wako Pure Chemical Industries. Tigecycline was purchased from the Tokyo Chemical Industry (Tokyo, Japan). The bacterial culture was adjusted to an OD₆₀₀ of 0.001 from the overnight cultures. MICs were monitored using a 2-fold dilution series for each antibiotic or disinfectant.

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Kamoshida G., Yamada N., Nakamura T., Yamaguchi D., Kai D., Yamashita M., Hayashi C., Kanda N., Sakaguchi M., Morimoto H., Sawada T., Okada T., Kaya Y., Takemoto N, & Yahiro K. (2022). Preferential Selection of Low-Frequency, Lipopolysaccharide-Modified, Colistin-Resistant Mutants with a Combination of Antimicrobials in Acinetobacter baumannii. Microbiology Spectrum, 10(5), e01928-22.

Publication 2022

LB broth Mueller-Hinton broth colistin meropenem amikacin ciprofloxacin ethanol SDS benzalkonium Tigecycline

Amikacin Antibiotic Antibiotics Bacterial Benzalkonium Ciprofloxacin Colistin Dilution Ethanol H2o2 Meropenem Mics Tigecycline

Corresponding Organization : National Center for Global Health and Medicine

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Variable analysis

independent variables

Antibiotic agents (colistin, meropenem, amikacin, ciprofloxacin)
Disinfectants (ethanol, H2O2, SDS, benzalkonium)
Tigecycline

dependent variables

Minimum Inhibitory Concentrations (MICs) of antibiotics and disinfectants

control variables

LB broth for antibiotics
Mueller-Hinton broth (BD Biosciences) for disinfectants
Bacterial culture adjusted to an OD600 of 0.001 from the overnight cultures
2-fold dilution series for each antibiotic or disinfectant

controls

No positive or negative controls were explicitly mentioned.

Annotations

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