For most experiments, Arabidopsis thaliana plants were grown in “Arabidopsis Mix” greenhouse potting soil (equal parts of SUREMIX [Michigan Grower Products Inc.], medium vermiculate and perlite; autoclaved once) or Redi-Earth soil (Sun Gro Horticulture) in air-circulating growth chambers for colonization of phyllosphere microbiota. Plants were grown under relative humidity set at 60%, temperature at 22°C, light intensity at 100 μE⋅m2⋅s and photoperiod at a 12 h light-12 h dark cycle. Five-week-old plants were used for bacterial inoculation and microbiota assays.
The wild-type accession Col-0, min7 fls2 erf cerk1 (mfec), ben3 (i.e., cad1–5 used in this study) and dde2–2/ein2–1/pad4–1/sid2–2 (deps) mutant derivatives were described previously1 (link),21 (link),32 (link) or in this study. The big2–1 (SALK_033446) and big2–2 (SALK_016558) T-DNA insertion mutants were obtained from the Arabidopsis Biological Resource Centre (ABRC) at The Ohio State University.
The wild-type accession Col-0, min7 fls2 erf cerk1 (mfec), ben3 (i.e., cad1–5 used in this study) and dde2–2/ein2–1/pad4–1/sid2–2 (deps) mutant derivatives were described previously1 (link),21 (link),32 (link) or in this study. The big2–1 (SALK_033446) and big2–2 (SALK_016558) T-DNA insertion mutants were obtained from the Arabidopsis Biological Resource Centre (ABRC) at The Ohio State University.
