OVA323–339 peptide was synthesized by the Emory University Microchemical Core Facility. Ovalbumin was from Worthington Biochemical Corporation and hen egg lysozyme was from Sigma-Aldrich. Some reagents were purchased: mouse IFN-γ (PeproTech), and lisinopril (Sigma). The PE-conjugated anti-CD69, PE-conjugated anti-CD80 (16–10A1), APC-conjugated anti-CD3ε, APC-conjugated anti-CD86 (GL-1) and the ELISA kits for IL-2, IFN-γ and IL-17A were all purchased from eBioscience. Alexa Fluor 488-conjugated Ovalbumin was from ThermoFisher. AF6–120.1 (anti-I-Ab),1G11 (anti-EEA1) and eBioH4A3 (anti-LAMP-1) were from eBioscience. A rabbit antiserum that recognizes mouse ACE was previously described (13 (link)). The FITC-conjugated mouse TCR Vβ Screening Panel was from BD Pharmingen. Flow cytometry stained samples were analyzed on CyAn ADP (Beckman Coulter) and data were analyzed with FlowJo software.
Protocol detail
Ovalbumin Peptide Immunization Protocol
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Ovalbumin
hen egg lysozyme
mouse IFN-γ
lisinopril
PE-conjugated anti-CD69
PE-conjugated anti-CD80 (16–10A1),
APC-conjugated anti-CD3ε
APC-conjugated anti-CD86 (GL-1)
ELISA kits for IL-2
IFN-γ
Alexa Fluor 488-conjugated ovalbumin
eBioH4A3 (anti-LAMP-1)
FITC-conjugated mouse TCR Vβ Screening Panel
CyAn ADP
Alexa fluor 488
Anti cd80
Antiserum
Cd3ε
Elisa
Fitc
Flow cytometry
Hen egg lysozyme
Ifn γ
Il 17a
Lamp 1
Lisinopril
Mouse
Mouse ifn γ
Ovalbumin
Peptide
Rabbit
Corresponding Organization : Zhejiang University
Other organizations : Cedars-Sinai Medical Center
Top 5 similar protocols
Variable analysis
independent variables
- OVA323–339 peptide
- Mouse IFN-γ
- Lisinopril
- Alexa Fluor 488-conjugated ovalbumin
- IFN-γ
- IL-17A
- Ovalbumin from Worthington Biochemical Corporation
- Hen egg lysozyme from Sigma-Aldrich
- PE-conjugated anti-CD69
- PE-conjugated anti-CD80 (16–10A1)
- APC-conjugated anti-CD3ε
- APC-conjugated anti-CD86 (GL-1)
- AF6–120.1 (anti-I-Ab)
- 1G11 (anti-EEA1)
- EBioH4A3 (anti-LAMP-1)
- Rabbit antiserum that recognizes mouse ACE
- FITC-conjugated mouse TCR Vβ Screening Panel
- Rabbit antiserum that recognizes mouse ACE
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