Microcell-mediated chromosome transfer in CHO cells
Corresponding Organization :
Other organizations : Tottori University, Tokyo University of Pharmacy and Life Sciences, Tokyo Metropolitan Institute of Medical Science
Variable analysis
- Transfer of 10MAC2-HR3 cells to CHO (Hprt-/-) cells
- Fusion of CHO cells with DT40(10MAC2-HR3)-derived microcells
- Selection of BS-resistant and EGFP-positive clones
- Identification of clones by PCR and FISH analysis
- BS and ouabain octahydrate (Sigma-Aldrich) used for selection of clones
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!