MMCT was performed as described previously27 (link). The 10MAC2-HR3 cells were transferred to CHO (Hprt−/−) cells. The CHO cells fused with DT40(10MAC2-HR3)-derived microcells were selected with 8 µg/mL of BS and ouabain octahydrate (Sigma-Aldrich), then we obtained BS-resistant and EGFP-positive clones. The clones were identified by PCR and FISH analysis.
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