Naive CD4+ T Cell Polarization by IL-33
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Corresponding Organization : Huazhong University of Science and Technology
Protocol cited in 1 other protocol
Variable analysis
- Exogenous rmIL-33 (10 ng/mL)
- PBS (as the control)
- Polarization of Treg
- Naïve CD4+ T cells (CD4+ CD25- CD44- CD62L+) cultured in 96-well plates
- Anti-CD3 mAb (5 μg/mL) and soluble anti-CD28 mAb (3 μg/mL) coated on the plates
- IL-2 (100 U/mL) presence in the culture medium
- Culture medium composition (RPMI-1640, 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL of Penicillin/Streptomycin)
- Culture duration (5 days)
- PBS
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