Naive CD4+ T Cell Polarization by IL-33

Liver naïve CD4+ T cells (CD4⁺ CD25⁻ CD44⁻ CD62L⁺) were sorted from the infected WT mice as previously described.31 (link) Naïve CD4+ T cells were then cultured (3×10⁵ cells/well) in 96-well plates coated with anti-CD3 mAb (5 μg/mL, eBioscience) and soluble anti-CD28 mAb (3 μg/mL, eBioscience) and kept in the presence of IL-2 (100 U/mL, eBioscience). Exogenous rmIL-33 (10 ng/mL) was added to investigate its ability to polarize Treg and PBS used as the control. Cells were cultured in RPMI-1640 Medium, 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL of Penicillin/Streptomycin for 5 days.

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Bai Y., Guan F., Zhu F., Jiang C., Xu X., Zheng F., Liu W, & Lei J. (2021). IL-33/ST2 Axis Deficiency Exacerbates Hepatic Pathology by Regulating Treg and Th17 Cells in Murine Schistosomiasis Japonica. Journal of Inflammation Research, 14, 5981-5998.

Publication 2021

anti-CD3 mAb soluble anti-CD28 mAb IL-2

Anti cd3 Cd25 Cd4 t cells Cd44 Cd62l Cells Cultured medium Fetal calf serum Glutamine Liver cells Mice Penicillin Streptomycin

Corresponding Organization : Huazhong University of Science and Technology

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Variable analysis

independent variables

Exogenous rmIL-33 (10 ng/mL)
PBS (as the control)

dependent variables

Polarization of Treg

control variables

Naïve CD4+ T cells (CD4+ CD25- CD44- CD62L+) cultured in 96-well plates
Anti-CD3 mAb (5 μg/mL) and soluble anti-CD28 mAb (3 μg/mL) coated on the plates
IL-2 (100 U/mL) presence in the culture medium
Culture medium composition (RPMI-1640, 10% fetal calf serum, 2 mM L-glutamine, 100 U/mL of Penicillin/Streptomycin)
Culture duration (5 days)

negative control

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