Cells were stained with monoclonal antibodies specific for CD138 (281-2; BioLegend) or IgM (331.12; eBioScience). Intracellular staining was performed using BD Cytofix/Cytoperm (BD Biosciences). Cell viability was determined by the addition of 1 μg/mL Propidium Iodide (PI; Sigma-Aldrich), 1 μg/mL FluoroGold (Sigma-Aldrich) or 1 μL/mL eFluoro-780 (eBioscience).
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