Flow Cytometry Immunophenotyping Protocol
Corresponding Organization :
Other organizations : University of Melbourne, Massachusetts General Hospital, Harvard University, Walter and Eliza Hall Institute of Medical Research, Cornell University
Variable analysis
- Monoclonal antibodies specific for CD138 (281-2; BioLegend)
- Monoclonal antibodies specific for IgM (331.12; eBioScience)
- Cell viability determined by the addition of Propidium Iodide (PI; Sigma-Aldrich)
- Cell viability determined by the addition of FluoroGold (Sigma-Aldrich)
- Cell viability determined by the addition of eFluoro-780 (eBioscience)
- Intracellular staining was performed using BD Cytofix/Cytoperm (BD Biosciences)
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