All data were acquired as previously described39 (link) with a 3 Tesla scanner (Philips Ingenia, Philips AG, Zurich, Switzerland) using a 16‐channel receive‐transmit head coil (dStream HeadSpine coil solution, Philips AG). From the anatomical evaluation, a 3D T1-weighted (T1W; TR 13 ms; TE 6 ms; FOV 130 mm; slice thickness 0.6 mm; Flip angle 8°) sequence was depicted for the registration with anatomical images. Following the anatomical scans, around one hour after induction of anaesthesia, rs-fMRI scans were acquired in all dogs.
A gradient-echo planar imaging (EPI) sequence was performed using the following protocol: TR 2.0 s; TE 30 ms; FOV 236 mm; slice thickness 3.0 mm; acquisition time 12.07 min. Phase encoding direction was anterior–posterior.
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