Flow cytometric gating was based on fluorescence minus one controls in cases where bimodal distribution was not apparent. To generate histogram comparisons of fluorescence intensity across samples run with the same cytometer settings on different days, we first performed batch correction with the function SwiftReg(70 (link)) on MATLAB_R2020b (The MathWorks, Inc., Natick, MA, USA). Comparisons were then performed with concatenations of all samples containing equal proportions of the cell type of interest. For comparison of two groups without matching, a Mann-Whitney test was performed. Comparisons of two groups with matching samples were performed with Wilcoxon matched-pairs signed rank tests. For comparisons of three or more groups, a Friedman test was performed. For paired comparisons across three or more groups, a mixed-effects analysis was performed. For all plots derived from flow cytometric data, no correction was made for multiple comparisons and all comparisons made in the statistical analysis are displayed. All statistical analyses were done using Prism 9.0.2 (GraphPad Software, San Diego, CA, USA). Statistical significance is indicated by one or more asterisks, and the number of asterisks shown correspond to p-values less than 0.05, 0.01, 0.001, or 0.0001, respectively.