Silage Analysis: Extraction and Quantification

The liquid extract was obtained by taking 10 g of silage sample, adding 90 mL of distilled water, tapping it for 2 min on a homogenizer tapper, and filtering it through four layers of coarse cotton cloth and filter paper. The prepared filtrates were used for measuring pH, ammonia nitrogen (NH₃-N), and organic acids. The pH was determined by a pH meter (Model: LEICI pH S-3C, Shanghai Yitian Scientific Instrument Co., Ltd., Shanghai, China). The content of lactic acid (LA) and acetic acid (AA) was measured by high performance liquid chromatography (Model: Waters e2695, Milford, MA, USA). The concentration of ammonia nitrogen (NH₃-N) was measured by the phenol-hypochlorous acid colorimetric method of Broderick and Kang [26 (link)].