Cell Proliferation Assay with ACPSVs

5 × 10⁴ HeLa cells/well were seeded overnight in 24-well culture dishes (Costar) in regular DMEM media containing 10% FBS and Penicillin–Streptomycin antibiotics. The next day, the adherent cells were treated with media alone or media containing 50 µl of purified ACPSVs, which were purified from either healthy or apoptotic culture supernatants or from solid tumors. The proliferation-inducing effect associated with ACPSV contents was determined by cell counts after growing recipient cells for 24 h at 37 °C in the presence of 5% CO₂, using a hemocytometer after trypsin digestion, as described^{4 (link)}.