Total RNA was isolated from the eKC and mKC sorted from Cx3cr1-CreERT2R26-tdT mice with the RNeasy Micro Kit (Qiagen, Hilden, Germany) according to manufacturer’s protocol. Quality and quantity of RNA was estimated with Bioanalyzer with Nano total RNA Chips (Agilent, Santa Clara, CA, USA), the RIN values exceeded 7.8 for all samples used for microarray measurement. Total RNA was amplified, fragmented and labeled with biotin using the GeneChip WT Pico Reagent Kit (Affymetrix, Santa Clara, CA, USA) and samples were hybridized to GeneChip Mouse ST Arrays 2.0 according to the manufacturer’s instructions. Probeset signal intensities were background-corrected and normalized using the RMA algorithm. Significantly regulated genes were identified using paired-T tests (pairing by animal, limma package of R/Bioconductor) with Benjamini-Hochberg correction for multiple testing. Genes regulated at least 1.5 fold with p < 0.1 were considered significant. The microarray analysis was done with CARMAweb 1.5 software38 (link).