Comprehensive Statistical Analysis of Biomarkers

For statistical analysis the SPSS package (IBM, UK) was utilized. As there were four participant groups and the bio-markers are all continuous we have used analysis of variance (ANOVA) to determine if there is evidence of differences across the groups. Statistical significance of the Q-PCR results was assessed by the ANOVA with Tukey HSD correction to evaluate the differences between means. This analysis was carried out on the deltaCT values before calculation of the transcript copy number (fold change). The graphical representation of the QPCR results represents the calculated transcript copy number and the statistics shown in the graphs represents analysis based on the deltaCT values. Results were considered significant at P<0.05. Statistical analysis of the Western blot data was carried out using Graphpad Prism6 software package (GraphPad Software, Inc., La Jolla, CA, USA). Statistical significance of the results was assessed by the Mann Whitney Test as described by Eaton et al [25 (link)].

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Brzeszczyńska J., Johns N., Schilb A., Degen S., Degen M., Langen R., Schols A., Glass D.J., Roubenoff R., Greig C.A., Jacobi C., Fearon K.C, & Ross J.A. (2016). Loss of oxidative defense and potential blockade of satellite cell maturation in the skeletal muscle of patients with cancer but not in the healthy elderly. Aging (Albany NY), 8(8), 1690-1702.

Publication 2016

SPSS package Graphpad Prism6

Western blot analysis

Corresponding Organization : University of Edinburgh

Other organizations : Novartis (Switzerland), Novartis (United States), Maastricht University, University of Birmingham

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Variable analysis

independent variables

Participant groups (4 groups)

dependent variables

Bio-markers (all continuous)

control variables

Not explicitly mentioned

controls

Positive control: Not mentioned
Negative control: Not mentioned

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