We analyzed the feces of 25 RP patients and 27 normal individuals to obtain 16S rRNA metagenomic data. Mean age (years) of the patients was 54.8 ± 2.8 (standard error of the means) and that of normal individuals was 52.8 ± 2.8. The male to female ratios were 7:18 in RP patients and 12:15 in normal individuals. In patients, mean age of disease onset was 44.8 ± 3.4 and mean disease duration (years) was 10.0 ± 1.6. The clinical data of the 25 RP patients were summarized in Table 1 .
We described here the study methods briefly. We extracted genomic DNA from fecal samples by treating them with achromopeptidase (Wako Pure Chemical Industries, Tokyo, Japan) [20 ]. We amplified the V1–V2 16S rRNA gene region by primers according to a procedure reported previously [21 (link)]. We purified (AMPure XP magnetic purification beads, Beckman Coulter, Tokyo, Japan), quantified (Agilent 2100 Bioanalyzer, Agilent Technologies Japan, Tokyo, Japan), and sequenced the amplicon libraries (Ion Torrent PGM, Life Technologies Japan, Tokyo, Japan).
We described here the study methods briefly. We extracted genomic DNA from fecal samples by treating them with achromopeptidase (Wako Pure Chemical Industries, Tokyo, Japan) [20 ]. We amplified the V1–V2 16S rRNA gene region by primers according to a procedure reported previously [21 (link)]. We purified (AMPure XP magnetic purification beads, Beckman Coulter, Tokyo, Japan), quantified (Agilent 2100 Bioanalyzer, Agilent Technologies Japan, Tokyo, Japan), and sequenced the amplicon libraries (Ion Torrent PGM, Life Technologies Japan, Tokyo, Japan).
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