Immunohistochemistry was performed as described previously.14 (link) Briefly, tissue slides were sequentially incubated with rabbit
anti-vinculin (1:500; Abcam) and HRP-conjugated goat anti-rabbit IgG antibodies
(1:1000; Abcam). Counterstaining was performed with hematoxylin. Slides were
observed under a DMR fluorescence microscope (Leica, Solms, Germany).
Results were analyzed independently by two expert pathologists who were blinded
to the clinical data. A semi-quantitative histopathology (H) score was obtained
from the staining intensity score (0, no staining; 1, weak staining; 2, moderate
staining; and 3, strong staining) × percentage score (0–300). An H-score lower
than the median was considered to be negative.
anti-vinculin (1:500; Abcam) and HRP-conjugated goat anti-rabbit IgG antibodies
(1:1000; Abcam). Counterstaining was performed with hematoxylin. Slides were
observed under a DMR fluorescence microscope (Leica, Solms, Germany).
Results were analyzed independently by two expert pathologists who were blinded
to the clinical data. A semi-quantitative histopathology (H) score was obtained
from the staining intensity score (0, no staining; 1, weak staining; 2, moderate
staining; and 3, strong staining) × percentage score (0–300). An H-score lower
than the median was considered to be negative.
