MLST and PFGE for CAZ/AVI-resistant K. pneumoniae

Seven conserved housekeeping genes—gapA, infB, mdh, pgi, phoE, rpoB, and tonB—were used to perform MLST (

http://bigsdb.pasteur.fr/klebsiella/klebsiella.html

). Clonal relatedness among CAZ/AVI-resistant K. pneumoniae isolates was established using XbaI - PFGE (TaKaRa). DNA fragments were separated with a CHEF DR III apparatus (Bio-Rad, Richmond, CA, USA). The molecular marker was Salmonella serotype Braenderup strain H9812. The isolates sharing >80% similarity were defined as the same PFGE cluster.22 (link)

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Li D., Liao W., Huang H.H., Du F.L., Wei D.D., Mei Y.F., Long D., Wan L.G., Liu Y, & Zhang W. (2020). Emergence of Hypervirulent Ceftazidime/Avibactam-Resistant Klebsiella pneumoniae Isolates in a Chinese Tertiary Hospital. Infection and Drug Resistance, 13, 2673-2680.

Publication 2020

CHEF DR III

Clonal Housekeeping genes K pneumoniae Klebsiella Molecular marker Pfge Salmonella Strain

Corresponding Organization : Nanchang University

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Variable analysis

independent variables

MLST (Multilocus Sequence Typing) using seven conserved housekeeping genes: gapA, infB, mdh, pgi, phoE, rpoB, and tonB
XbaI-PFGE (Pulsed-Field Gel Electrophoresis) to establish clonal relatedness among CAZ/AVI-resistant K. pneumoniae isolates

dependent variables

Clonal relatedness among CAZ/AVI-resistant K. pneumoniae isolates

control variables

CHEF DR III apparatus (Bio-Rad, Richmond, CA, USA) for DNA fragment separation
Salmonella serotype Braenderup strain H9812 as the molecular marker

controls

The isolates sharing >80% similarity were defined as the same PFGE cluster, which serves as a control for determining clonal relatedness

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