ChIP assay was performed as previously described (10 (link), 30 (link)). The PCR primers used were: PKMYT1 promoter, 5′-TTATGGACCCAAACACTACGC-3′ and 5′-CGCCAAAAATTCCAAACC-3′; and BLM promoter, 5′-GGCTGAAACAGAAGCATGG-3′ and 5′-TCACCCGTACCCCTCTACAC-3′. Antibodies used in this study were: IgG (sc-2027, Santa Cruz Biotechnology), GAL4 (IgG2A negative control) (Santa Cruz Biotechnology), and MYCN (sc-53993, Santa Cruz Biotechnology).
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