Animal experiments were approved by the Committee on Ethics of Animal Experiments, Sun Yat-sen University and conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals published by the US National Institutes of Health (NIH Publication No. 85–23, revised 1996).
Male Sprague-Dawley (SD) rats weighing 160–180g were obtained from Sun Yat-sen University. A two-step 5/6 NE model of CRF was used as described in our previous study [28 (link)]. Rats were randomly divided into three groups: sham-operated group, NE group and NE+apocynin group. Rats in NE+apocynin group were fed with 1.5 mM apocynin (Sigma-Aldrich, St. Louis, US) in drinking water [30 (link)]. 8 weeks after surgery, SBP, DBP and heart rate of the animals were measured by a tail-cuff method (BP-98A, Softron, Tokyo, Japan). Plasma was obtained by tail vein at 8 weeks for determining the levels of creatinine (model 7600–010, HITACHI automatic analyzer, Tokyo, Japan) and 14, 15-EET levels (Detroit R&D Inc.). Cardiac tissues were harvested at sacrifice for histological and molecular investigations.
Male Sprague-Dawley (SD) rats weighing 160–180g were obtained from Sun Yat-sen University. A two-step 5/6 NE model of CRF was used as described in our previous study [28 (link)]. Rats were randomly divided into three groups: sham-operated group, NE group and NE+apocynin group. Rats in NE+apocynin group were fed with 1.5 mM apocynin (Sigma-Aldrich, St. Louis, US) in drinking water [30 (link)]. 8 weeks after surgery, SBP, DBP and heart rate of the animals were measured by a tail-cuff method (BP-98A, Softron, Tokyo, Japan). Plasma was obtained by tail vein at 8 weeks for determining the levels of creatinine (model 7600–010, HITACHI automatic analyzer, Tokyo, Japan) and 14, 15-EET levels (Detroit R&D Inc.). Cardiac tissues were harvested at sacrifice for histological and molecular investigations.
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