Drosophila genetics and molecular tools

Flies were raised at 25°C, on regular fly food. Sik2^Δ41 and UAS-Sik2^K170M lines were kindly provided by Jongkyeong Chung (Seoul, Korea) [9 (link)]. Sensitized and eyeful lines were kindly provided by Bassem Hassan (Paris, France) [2 (link)]. UAS-Dicer2; ey-GAL4, lGMR-Gal4 / CyO was a kind gift from Claude Desplan (New York, USA) [40 (link)].
The following fly lines were obtained from the Bloomington Drosophila Stock Center: w¹¹¹⁸ (3605), UAS-Notch (26820), UAS-Delta (5612), UAS-Ser (5815), UAS-Fringe (5831), UAS-Delta.DN (26697), lGMR-GAL4 (8121), ey-GAL4 (5535), elav-GAL4 (458), mirr-GAL4 (29650), sca-GAL4 (6479), neoFRT42D, GMR-myr-GFP (7110), neoFRT42D, GMR-hid / CyO; ey-GAL4, UAS-FLP (5251), actin-GAL4 (25374). Following RNAi lines were supplied by the VDRC Stock Center: Sik3-RNAi (107458), Sik2-RNAi (103739), Ser-RNAi (108348), Delta-RNAi (3720), Fringe-RNAi (51977).

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Şahin H.B., Sayın S., Holder M., Buğra K, & Çelik A. (2020). Salt inducible kinases as novel Notch interactors in the developing Drosophila retina. PLoS ONE, 15(6), e0234744.

Publication 2020

w1118 UAS-Ser

Actin Drosophila Food Rnai

Corresponding Organization : Boğaziçi University

Other organizations : The Francis Crick Institute

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