Bone marrow lineagec-Kit+ (LK) HSPCs were separated by EasySep magnets (cat #: 18000, STEMCELL Technologies, Vancouver, BC, Canada) using lineage-negative and c-Kit-positive selection kits (cat #s: 19856 and 18757, STEMCELL Technologies, Vancouver, BC, Canada). To determine the effects of PGN on HSPCs ex vivo, the cells were pretreated with purified PGN (100 μg/mL), for 2 h in culture medium [12 (link)].
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