Measuring Gene Expression Regulation in Immune Cells

Electrophoretic mobility shift assays (EMSA) were performed using nuclear extract from Jurkat, LCL, K562, HUVEC cell lines and peripheral blood mononuclear cells (PBMCs) from healthy individuals (supplementary methods). Expression quantitative trait locci (QTLs) were analysed using GTEx datasets,25 (link) and IL19 (R&D Systems), IL10 (Mesoscale Discovery) and aPL levels were measured (supplementary methods).12 (link)

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Leonard D., Svenungsson E., Dahlqvist J., Alexsson A., Ärlestig L., Taylor K.E., Sandling J.K., Bengtsson C., Frodlund M., Jönsen A., Eketjäll S., Jensen-Urstad K., Gunnarsson I., Sjöwall C., Bengtsson A.A., Eloranta M.L., Syvänen A.C., Rantapää-Dahlqvist S., Criswell L.A, & Rönnblom L. (2018). Novel gene variants associated with cardiovascular disease in systemic lupus erythematosus and rheumatoid arthritis. Annals of the Rheumatic Diseases, 77(7), 1063-1069.

Publication 2018

IL19 IL10

Cell lines Electrophoretic mobility shift assays Il10 Il19 Peripheral blood mononuclear cells

Corresponding Organization :

Other organizations : Uppsala University, Science for Life Laboratory, Center for Rheumatology, Karolinska University Hospital, Karolinska Institutet, Umeå University, University of California, San Francisco, Linköping University, Skåne University Hospital, AstraZeneca (Poland), Integrated Cardio Metabolic Centre, Stockholm South General Hospital

Top 5 similar protocols

Variable analysis

independent variables

Cell lines (Jurkat, LCL, K562, HUVEC)
Peripheral blood mononuclear cells (PBMCs) from healthy individuals

dependent variables

Electrophoretic mobility shift assay (EMSA) results
Expression quantitative trait locci (eQTLs) from GTEx datasets
Levels of IL19 (R&D Systems), IL10 (Mesoscale Discovery), and aPL

control variables

Not explicitly mentioned

controls

Positive control: Not specified
Negative control: Not specified

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!