Open Field and Contextual Fear Conditioning Protocols
Corresponding Organization :
Other organizations : Yale University, New York University
Variable analysis
- Exposure to open field arena
- Contextual fear conditioning (exposure to fear conditioning chamber, shock-tone pairing)
- Distance traveled in the open field arena
- Time spent in the inner 15 cm-area of the open field arena
- Freezing behavior during contextual fear conditioning
- Size of open field arena (40 × 40 cm)
- Illumination level in the open field arena (60 lx)
- Duration of open field test (5 min)
- Fear conditioning chamber (Med Associates' fear conditioning chamber, Cat # VFC-008)
- Grid floor in the fear conditioning chamber
- Presence of 2% lemon scent in the fear conditioning chamber
- Shock-tone pairing parameters (30 s discontinued tones at 7500 Hz, 80 dB; 1 s 0.65 mA foot shock)
- Variable inter-trial interval for shock-tone pairing (30–180 s)
- Duration of contextual fear conditioning test (300 s)
- None specified
- None specified
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