To assess the influence of DEK overexpression or depletion in GC cell lines on metastatic activity, the in vitro Transwell assay (Becton-Dickinson, Franklin Lakes, NJ, USA) was employed. After adjusting the density to 1 × 105 cells/100 μL serum-free RPMI, cells were added to the upper chamber and a Matrigel-coated (invasion assay) insert used to assess invasive capability, as described previously [46 (link)].
Free full text: Click here