Before the sacrifice, blood was drawn from the tail vein, and the systolic blood pressure was measured afterwards. A total of 800 µL of blood were drawn into tubes containing L-heparin (Sarstedt, Nümbrecht, Germany). Following a 10 min, 3000 rpm centrifugation of the blood samples, the plasma was extracted and kept at −80 °C. Blood parameters (Supplementary Table S1 ) were evaluated as previously described [20 (link),21 (link),22 (link),23 (link),24 (link)]. Leptin concentration was evaluated by the colorimetric method using a specific kit (Rat Leptin ELISA Kit, Abcam ab100773, Cambridge CB2 0AX, UK).
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