Immunofluorescence Staining of E-Cadherin

Immunofluorescence studies were performed as described previously (Ma et al, 2010 (link)), with an anti-E-cadherin primary antibody (#3195, 1 : 200 dilution; Cell Signaling Technology, Danvers, MA, USA) and an Alexa Fluor-647-conjugated anti-rabbit secondary antibody (#4414, 1 : 1000 dilution; Cell Signaling Technology). For staining of nuclei, DAPI was used.

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Miyo M., Yamamoto H., Konno M., Colvin H., Nishida N., Koseki J., Kawamoto K., Ogawa H., Hamabe A., Uemura M., Nishimura J., Hata T., Takemasa I., Mizushima T., Doki Y., Mori M, & Ishii H. (2015). Tumour-suppressive function of SIRT4 in human colorectal cancer. British Journal of Cancer, 113(3), 492-499.

Publication 2015

anti-E-cadherin primary antibody Alexa Fluor-647-conjugated anti-rabbit secondary antibody

Alexa fluor 647 Anti antibody Cadherin Dapi Dilution Immunofluorescence Nuclei Rabbit

Corresponding Organization :

Other organizations : Osaka University

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Variable analysis

independent variables

Primary antibody: anti-E-cadherin antibody (#3195, 1 : 200 dilution; Cell Signaling Technology, Danvers, MA, USA)

dependent variables

E-cadherin expression (measured via immunofluorescence)

control variables

Secondary antibody: Alexa Fluor-647-conjugated anti-rabbit secondary antibody (#4414, 1 : 1000 dilution; Cell Signaling Technology)
Nuclear staining: DAPI

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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