Mitochondrial DNA (mtDNA; #61014) was visualized on an Olympus FV3000 Confocal Laser Scanning Microscope as previously described28 (link). Ten images containing more than 300 cells from three independent experiments were analyzed with Imaris 9.1 software. mKeima-Cox8-treated HeLa cells were prepared in Dr. HM Shen’s laboratory.
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