Quantitative Histomorphometric Analysis of Peri-Implant Bone

After implant removal, bone specimens were immediately fixed in 10% neutral buffered formalin and maintained at room temperature. Then, they were decalcified for at least 72 h in a mixture of formic and hydrochloric acids (BIODEC R, Bio Optica Milano, Italy), sectioned along the longitudinal implant axis and embedded in paraffin. From each bone portion, sections (5 µm) were obtained and stained with hematoxylin-eosin (H&E) for optical microscopy. The following parameters were analyzed in peri-implant tissue corresponding to 2 mm around each implant site: (1) maximum length of the newly formed bone (mm) measured from the implant profile; (2) bone tissue percentage; (3) number of osteoblasts. All data represent the mean of 10 fields.
Furthermore, sections stained with H&E were digitized using the Hamamatsu’s Nanozoomer 2 scanner (Aperio ImageScope, Buccinasco, Milano, Italy). Areas of new bone deposition and fibrous tissue were marked using an imaging computer software (Aperio ImageScope, Buccinasco, Milano, Italy), analyzed according to a protocol proposed by Han, J.-M. et al. [19 (link)], and expressed as total surface area.

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Schierano G., Baldi D., Peirone B., Mauthe von Degerfeld M., Navone R., Bragoni A., Colombo J., Autelli R, & Muzio G. (2021). Biomolecular, Histological, Clinical, and Radiological Analyses of Dental Implant Bone Sites Prepared Using Magnetic Mallet Technology: A Pilot Study in Animals. Materials, 14(22), 6945.

Publication 2021

BIODEC R

Axis Bone Bone tissue Embedded paraffin Eosin Fibrous Formalin Formed bone Hydrochloric acids Optical microscopy Osteoblasts Tissue

Corresponding Organization :

Other organizations : University of Turin, University of Genoa

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Variable analysis

independent variables

Removal of implant

dependent variables

Maximum length of the newly formed bone (mm) measured from the implant profile
Bone tissue percentage
Number of osteoblasts
Total surface area of new bone deposition and fibrous tissue

control variables

Bone specimens were immediately fixed in 10% neutral buffered formalin and maintained at room temperature
Bone specimens were decalcified for at least 72 h in a mixture of formic and hydrochloric acids (BIODEC R, Bio Optica Milano, Italy)
Bone specimens were sectioned along the longitudinal implant axis and embedded in paraffin
Sections (5 µm) were obtained and stained with hematoxylin-eosin (H&E) for optical microscopy

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