Ratiometric Lysosomal pH Measurement
Corresponding Organization :
Other organizations : University College London, National Institute of Neurological Disorders and Stroke, National Institutes of Health, UK Dementia Research Institute, Augusta University, University of Georgia, University of Cambridge, University of Oxford, George Washington University
Variable analysis
- Genotype (WT, GRN-KO)
- Lysosomal pH
- Cell type (i3Neurons)
- Cell culture conditions (96-well dishes, neuronal media)
- Dextran labeling (50 μg/mL Oregon Green-488 dextran, 50 μg/mL Alexa Fluor-555 red dextran)
- Incubation times (4 hours for dextran loading, overnight chase)
- Imaging conditions (live cell spinning disk confocal microscopy, Opera Phenix HCS System)
- Calibration (physiological buffers of known pH 4-8 with 10 μg/mL nigericin)
- Physiological buffers of known pH (4-8) containing 10 μg/mL nigericin for calibration curve
- Not explicitly mentioned
Annotations
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