A549 and THP-1 cells were cultured in DMEM and RPMI1640, respectively, supplemented with 10% (fetal calf serum) FCS as described [57 (link)]. Human small airway epithelial cells (HSAEpC) were from PromoCell (Heidelberg, Germany) and were cultured in MV2 medium (Promocell, Heidelberg, Germany) as described by the manufacturer. Cells were grown until confluency with a fully supplemented medium for 48 h. For inhibitor pre-incubation, cells were washed with PBS and received serum-free medium with GI254023 (10 μM) for 30 min.
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