Live-cell immunofluorescence was done as described (Pathak et al., 2013 (link)) with modifications. Briefly, cells were grown in CTT overnight to mid-log phase and harvested at 8000 × g for 2 min. Cell pellets were washed once in TPM and then ~ 9×108 cells were incubated in 1 ml of TPM with 2% BSA for 30 min. Following incubation, primary antibody (1:500 dilution) was added and further incubated for 30 min. Cell pellets were then resuspended in TPM containing 2% BSA with a fluorophore conjugated secondary antibody (1:300; Alexa Fluor 488-conjugated donkey anti-rabbit IgG or Alexa Fluor 594-conjugated donkey anti-rabbit IgG; Jackson ImmunoResearch) and incubated for 30 min in the dark. All the incubations were done at room temperature (RT) with gentle end-to-end rocking. Finally, cells were washed four times in TPM and spotted on 1% agarose pads (w/v) in TPM and imaged using a phase contrast 100× objective lens on a Nikon E800 fluorescence microscope.