Western Blot Analysis of CASP9 Protein

Tissues were lysed with RIPA lysis buffer containing protease and phosphatase inhibitors (Thermo Fisher Scientific, United States), and protein lysates were resolved by SDS-PAGE gels (Thermo Fisher Scientific, United States), blotted onto PVDF membranes (Roche, Switzerland) for analysis, and incubated overnight at 4°C with anti-CASP9 antibody (1:1,000 dilution; proteintech, China) and anti-ACTB antibody (1:2000 dilution; Cell Signaling Technology, United States). Data analysis was performed using Image J software.

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Gao J., Wang D., Yang Q., Tang M., Du J., He L, & Liu W. (2023). The signature of pyroptosis-related gene prognostic and immune microenvironment in adrenocortical carcinoma. Frontiers in Molecular Biosciences, 10, 1131402.

Publication 2023

RIPA lysis buffer protease and phosphatase inhibitors SDS-PAGE gels PVDF membranes anti-CASP9 antibody anti-ACTB antibody

Anti antibody Buffer Casp9 Dilution Gels Inhibitors Membranes Phosphatase Protease Protein Pvdf Ripa Sds page Tissues

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Variable analysis

independent variables

RIPA lysis buffer containing protease and phosphatase inhibitors
Anti-CASP9 antibody (1:1,000 dilution)
Anti-ACTB antibody (1:2000 dilution)

dependent variables

Protein lysates resolved by SDS-PAGE gels
Protein lysates blotted onto PVDF membranes for analysis

control variables

Incubation of membranes overnight at 4°C

controls

Positive control: Not specified
Negative control: Not specified

Annotations

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