Confocal microscopy was used to capture images of dendrites from the CA1 region of the hippocampus, based on previously described methods (75 (link), 78 (link)). A blinded experimenter performed all imaging. Images were captured with a Nikon (Tokyo, Japan) Ti2 C2 confocal microscope. The experimenter identified secondary dendrites from dye-impregnated neurons and captured three-dimensional z-stacks of those meeting the following criteria: (1 (link)) within 80 μm working distance of microscope; (2 (link)) relatively parallel with the surface of the coronal section; (3 (link)) no overlap with other branches; (4 (link)) minimum of 50 μm from the soma; (5 (link)) maximum of 110 μm from the soma. For each dendrite, z-stacks were captured with a 60X oil-immersion objective (Nikon Plan Apo, N.A. 1.40) using the following parameters: z-step: 0.1 μm; image size: 1024 × 512 px (0.04 μm x 0.04 μm x 0.1 μm); zoom: 4.8x; line averaging: 4; acquisition rate: 1 frame/sec. Captured images were deconvolved using Huygens Deconvolution System (16.05, Scientific Volume Imaging, the Netherlands) and the following settings: GMLE; maximum iterations: 10; signal to noise ratio: 15; quality: 0.003. Deconvolved images were saved in .tif format.