A standard protocol was used for immunoblot analysis [51 (link)]. The commercial antibodies used in this study are listed in Supplementary Table 2 . For chemiluminescent detection, blots were incubated with horseradish peroxidase-conjugated secondary antibodies (1:5000; Cayman, Ann Arbor, MI, USA) at room temperature, followed by enhanced chemiluminescence detection according to the manufacturer's protocol (Millipore, Billerica, MA, USA). All blots were also immunoblotted for β-actin (Sigma, St. Louis, MO, USA) to demonstrate equal loading of protein samples. All immunoblotting experiments were repeated at least 3 times.
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