Filtration Properties of Ventriculus-Posterior Midgut Connection

To test the filtration properties of the ventriculus-posterior midgut connection, polystyrene FMs of 50 nm (Polysciences) and a fluorescent tracer dye (Alexa Fluor 555, Thermo Fisher Scientific) were delivered using the method described above. In addition, fluorescein-labeled dextran of various sizes (500, 40, and 4 kDa, MilliporeSigma) were delivered to adult female mites as mixtures with 6% blue dye (erioglaucine; McCormick, Sparks Glencoe, MD) using the leaf coating method (Suzuki et al., 2017a (link)). To ensure the intactness of fluorescein-labeled dextran, the fluorescein-12-UTP (Roche) was delivered as a control. In both delivery systems, mites were allowed to feed for 24 h and fluorescence was visualized using an epifluorescence Zeiss Axioplan II microscope fitted with a FITC filter cube. Images were taken using an AxioCam Color HRc CCD Camera 412-312.

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Bensoussan N., Zhurov V., Yamakawa S., O'Neil C.H., Suzuki T., Grbić M, & Grbić V. (2018). The Digestive System of the Two-Spotted Spider Mite, Tetranychus urticae Koch, in the Context of the Mite-Plant Interaction. Frontiers in Plant Science, 9, 1206.

Publication 2018

Alexa Fluor 555 fluorescein-12-UTP Axioplan II microscope AxioCam Color HRc CCD Camera 412-312

Adult female Alexa fluor 555 Delivery Erioglaucine Filtration Fitc Fluorescein Fluorescein dextran Fluorescence Fluorescent dye Leaf Microscope Mites Polystyrene Ventriculus

Corresponding Organization : Tokyo University of Agriculture and Technology

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Variable analysis

independent variables

Polystyrene FMs of 50 nm (Polysciences)
Fluorescent tracer dye (Alexa Fluor 555, Thermo Fisher Scientific)
Fluorescein-labeled dextran of various sizes (500, 40, and 4 kDa, MilliporeSigma)
6% blue dye (erioglaucine; McCormick, Sparks Glencoe, MD)

dependent variables

Filtration properties of the ventriculus-posterior midgut connection

control variables

Fluorescein-12-UTP (Roche) was delivered as a control

controls

Positive control: Fluorescein-12-UTP (Roche) was delivered to ensure the intactness of fluorescein-labeled dextran
Negative control: Not mentioned

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