Isolation and Characterization of Stem Cells from Apical Papilla

A few sound mandibular 3rd molars (n = 12) were collected from young patients (18 to 20 years old) in the Oral and Maxillofacial Surgery Department, Sun Yat-sen University. The experiment protocol of the current research was proven by the Ethical Review Committee of Sun Yat-sen University. Freshly extracted third molars were rinsed by PBS, and the apical papillae were isolated by surgical instruments. Sequentially, SCAP were minced and treated with enzymatic digestion according to our previous reports [4 (link), 6 (link)]. Osteogenic and adipogenic differentiation of SCAP was analyzed by alizarin red staining and Oil Red staining, respectively. The SCAP phenotypic markers, such as STRO-1 (Santa Cruz, Delaware, CA), CD146 (BD, Pharmingen, USA), CD24 (BD, Pharmingen, USA), and CD45 (BD, Pharmingen, USA), were examined by flow cytometry.

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Zhang W., Zhang X., Li J., Zheng J., Hu X., Xu M., Mao X, & Ling J. (2018). Foxc2 and BMP2 Induce Osteogenic/Odontogenic Differentiation and Mineralization of Human Stem Cells from Apical Papilla. Stem Cells International, 2018, 2363917.

Publication 2018

STRO-1 CD146

Adipogenic Digestion Enzymatic Ethical review Flow cytometry Mandibular Molars Osteogenic Patients Phenotypic Sound Surgical instruments Third molars

Corresponding Organization : Sun Yat-sen University

Other organizations : Jinan University

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Variable analysis

independent variables

Enzymatic digestion treatment of SCAP

dependent variables

Osteogenic differentiation of SCAP (analyzed by alizarin red staining)
Adipogenic differentiation of SCAP (analyzed by Oil Red staining)
SCAP phenotypic markers (STRO-1, CD146, CD24, CD45 examined by flow cytometry)

control variables

Freshly extracted third molars from young patients (18 to 20 years old) in the Oral and Maxillofacial Surgery Department, Sun Yat-sen University
Rinsing of freshly extracted third molars with PBS
Isolation of apical papillae by surgical instruments

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