The myoblasts were incubated with paxilline (1, 5, 10, and 100 μM) or staurosporine (0.1 and 1 μM; positive control) for 16 h in high-serum growth medium. The cells were then collected, washed, and stained with 7-amino actinomycin D (7-AAD) to label DNA in non-viable cells as described.19 (link), 64 (link) The data were acquired with a Canto II flow cytometer and analyzed using FlowJo Version 7.
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