CAR-T cells were cocultured with various target cells as mentioned above for 12 h, followed by the addition of Golgi-Stop (BioLegend, #420701), and incubated for an additional 4 h to prevent cytokine secretion. Cells were harvested and stained for IFN-γ intracellularly as described previously.22 (link)
Protocol detail
Intracellular IFN-γ Assay for CAR-T Cells
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Corresponding Organization : Key Laboratory of Guangdong Province
Other organizations : General Hospital of Guangzhou Military Command
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Variable analysis
independent variables
- Target cells (various)
- IFN-γ production
- Incubation time (12 h + 4 h)
- Golgi-Stop addition
- Positive control: Not explicitly mentioned.
- Negative control: Not explicitly mentioned.
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