VACUETTE® K3EDTA tubes (2 mL) (Greiner Bio-One International GmbH, Kremsmünster, Austria) were used for LCT C/T-13910 genotyping. Genomic DNA was extracted from 200 μL EDTA blood with the Nucleic Acid Isolation Kit I on the MagNA Pure Compact Instrument (Roche Diagnostics, Vienna, Austria) according to the manufacturer’s instructions. Genotyping for the LCT C/T-13910 polymorphism was performed by real-time PCR and melting curve analysis (LCT T-13910C ToolSet™ [Roche Diagnostics]) on a LightCycler 2.0 Instrument (Roche Diagnostics) [21 (link)].
Fasting blood samples were drawn into VACUETTE® LH lithium tubes (4 mL) and centrifuged at 2000 x g for 10 minutes. HCY (normal range: 3.2–10.7 μmol/L), FA (normal range 3.1–17.5 ng/mL) and vitamin B12 (normal range: 182–625 pg/mL) measurements were performed by competitive chemiluminescent immunoassays on a Dimension Vista® 1500 System (Siemens Healthcare GmbH; Vienna, Austria). Creatinine (male: 0.7–1.3 mg/dL; female: 0.55–1.02 mg/dL) was measured using an enzymatic method applied on a Roche Cobas Mira (Roche Diagnostics, Vienna, Austria). The eGFR (normal: >70 mL/min/1.73m2) was calculated applying the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation [22 (link)].
Fasting blood samples were drawn into VACUETTE® LH lithium tubes (4 mL) and centrifuged at 2000 x g for 10 minutes. HCY (normal range: 3.2–10.7 μmol/L), FA (normal range 3.1–17.5 ng/mL) and vitamin B12 (normal range: 182–625 pg/mL) measurements were performed by competitive chemiluminescent immunoassays on a Dimension Vista® 1500 System (Siemens Healthcare GmbH; Vienna, Austria). Creatinine (male: 0.7–1.3 mg/dL; female: 0.55–1.02 mg/dL) was measured using an enzymatic method applied on a Roche Cobas Mira (Roche Diagnostics, Vienna, Austria). The eGFR (normal: >70 mL/min/1.73m2) was calculated applying the Chronic Kidney Disease Epidemiology Collaboration (CKD-EPI) equation [22 (link)].
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