Human dermal LECs and adipose-derived mesenchymal stem cells (ASCs) were obtained from PromoCell (Heidelberg, Germany) and stained in chamber slides for Prox1, Ki67 (Abcam), p-AKT (Cell Signaling Technology, Danvers, MA, USA) and VEGFR-3 (EMD Millipore, Billerica, MA, USA). Cell signal intensity was quantified using MetaMorph (Molecular Devices, Sunnyvale, CA, USA). Western blot analysis for VEGFR-3 (Abcam), p-AKT and p-eNOS (Cell Signaling Technology) was performed with total cellular protein harvested from LECs and quantified with NIH Image J.19 (link)
Protocol detail
Quantifying LEC and ASC Signaling Dynamics
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Adipose derived mesenchymal stem cells
Ascs
Cellular
Devices
Enos
Human
Lecs
Protein
Vegfr 3
Western blot analysis
Corresponding Organization :
Other organizations : Memorial Sloan Kettering Cancer Center
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Variable analysis
independent variables
- Cell type (human dermal LECs and adipose-derived mesenchymal stem cells (ASCs))
- Prox1 expression
- Ki67 expression
- P-AKT expression
- VEGFR-3 expression
- VEGFR-3 protein levels
- P-AKT protein levels
- P-eNOS protein levels
- Not explicitly mentioned
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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