Cell Viability Assay of DACC-Coated Dressing

Cell viability after contact with either the DACC-coated dressing or the uncoated reference was determined [21 (link)]. In brief, cells were harvested through trypsin-EDTA (Gibco) treatment, seeded into 4-well culture slides (BD Biosciences) at a density of 40,000 cells/cm², and cultured for 48 h to confluence. Stress controls and material samples were placed directly onto the cell layers. DMEM alone served as untreated (medium) controls. Cells were incubated for 1, 8, 24, and 48 h. Subsequently, the number of viable, active cells was determined using the photometric MTT assay (MTT Cell Proliferation Assay Kit, Invitrogen) according to the manufacturer’s recommendations. The absorbance was measured at 580 nm using a microplate photometer (POLARstar Galaxy, BMG Labtech). The number of viable cells was calculated as percentage of the medium control.