Agarose, diluted trypsin (0.25%),
RIPA buffer, N-tert-butyl-α-phenylnitrone
(PBN), N,N′-dimethylthiourea
(DMTU), and 1-palmitoyl-sn-glycero-3-phosphocholine
(LysoPC(16:0)) were purchased from Millipore Sigma (St. Louis MO).
Hydrogen peroxide, Pierce’s gold standard BCA assay, trypsin,
tris base, hydrochloric acid, acetone, formic acid, Pierce colorometric
peptide quantification assay, MS grade 0.1% formic acid in water,
MS grade 0.1% formic acid in acetonitrile, and MS grade water were
purchased from Thermo Fisher Scientific (Waltham, MA). McCoy’s
5A, fetal bovine serum (FBS),l -glutamine, phosphate-buffered
saline (PBS), and 0.05% porcine trypsin with 0.1% EDTA were purchased
from Gibco (Gaithersburg, MD). Iodoacetamide (IAA) was acquired from
ACROS Organics. Dithiothreitol (DTT) was purchased from American Bio
(Canton, MA). Dimethyl sulfoxide (DMSO) was purchased from Invitrogen
(Carlsbad, CA).
RIPA buffer, N-tert-butyl-α-phenylnitrone
(PBN), N,N′-dimethylthiourea
(DMTU), and 1-palmitoyl-sn-glycero-3-phosphocholine
(LysoPC(16:0)) were purchased from Millipore Sigma (St. Louis MO).
Hydrogen peroxide, Pierce’s gold standard BCA assay, trypsin,
tris base, hydrochloric acid, acetone, formic acid, Pierce colorometric
peptide quantification assay, MS grade 0.1% formic acid in water,
MS grade 0.1% formic acid in acetonitrile, and MS grade water were
purchased from Thermo Fisher Scientific (Waltham, MA). McCoy’s
5A, fetal bovine serum (FBS),
saline (PBS), and 0.05% porcine trypsin with 0.1% EDTA were purchased
from Gibco (Gaithersburg, MD). Iodoacetamide (IAA) was acquired from
ACROS Organics. Dithiothreitol (DTT) was purchased from American Bio
(Canton, MA). Dimethyl sulfoxide (DMSO) was purchased from Invitrogen
(Carlsbad, CA).
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