Murine Immune Cell Profiling by Flow Cytometry

Cells were released from culture plates using TrypLE and were stained after Fc-block (Invitrogen) with a panel of directly fluorochrome-conjugated mAbs against the following murine molecules (clone; source): Axl (R&D; Minneapolis, MN); CD4 (L3T4; eBioscience; San Diego, CA); CD8 (CD8b.2; Biolegend; San Diego, CA); CD11b (M1/70; eBioscience); CD11c (N418; eBioscience); CD19 (MCA1439F; AB Serotec); CD44 (IM7; eBioscience); CD45 (30-F11; BD); CD80 (16-10A1; BioLegend); CD206 (C068C2; BioLegend); Ly6C (AL-21; BD); Ly6G (1A8; BD, Franklin Lakes, NJ); Mertk (R&D). Experiments were performed on an LSR II flow cytometer and data were analyzed as previously described (25 (link)).

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McCubbrey A.L., Nelson J.D., Stolberg V.R., Blakely P.K., McCloskey L., Janssen W.J., Freeman C.M, & Curtis J.L. (2015). miR-34a negatively regulates efferocytosis by tissue macrophages in part via SIRT1. Journal of immunology (Baltimore, Md. : 1950), 196(3), 1366-1375.

Publication 2015

Fc-block CD4 (L3T4; CD11b (M1/70; CD11c (N418; CD44 (IM7; CD45 (30-F11; CD80 (16-10A1; CD206 (C068C2; Ly6C (AL-21; Ly6G (1A8; Mertk

Cd11b Cd44 Cells Clone Fluorochrome Mabs Mertk Murine

Corresponding Organization : VA Ann Arbor Healthcare System

Other organizations : University of Colorado Denver, University of Colorado Anschutz Medical Campus, National Jewish Health

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Variable analysis

independent variables

TrypLE
Fc-block (Invitrogen)
Directly fluorochrome-conjugated mAbs against the following murine molecules: Axl, CD4, CD8, CD11b, CD11c, CD19, CD44, CD45, CD80, CD206, Ly6C, Ly6G, Mertk

dependent variables

Staining pattern of murine molecules

control variables

Cell culture plates
LSR II flow cytometer
Analysis as previously described (25)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

Annotations

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