Male and female C57BL/6NCrSlc (B6N) mice embryos treated as normal phenotype and
B6N-XYPOS mice embryos [32 (link)] were used,
and all of their dams were maintained in 40.5 × 20.5 × 18.5-cm ventilated cages (Sealsafe
Plus Mouse, Tecniplast, Buguggiate, Italy) under controlled temperature (23 ± 2°C),
humidity (50 ± 10%) and ventilation (75 times/hr) on a 14-hr light/10-hr dark cycle at the Kobe University Life-Science Laboratory with
ad libitum access to water and a pellet diet (DC-8, Clea Japan, Tokyo,
Japan). This study was approved by the Institutional Animal Care and Use Committee
(Permission #25-06-03) and carried out according to the Kobe University Animal
Experimental Regulations.
B6N-XYPOS mice embryos [32 (link)] were used,
and all of their dams were maintained in 40.5 × 20.5 × 18.5-cm ventilated cages (Sealsafe
Plus Mouse, Tecniplast, Buguggiate, Italy) under controlled temperature (23 ± 2°C),
humidity (50 ± 10%) and ventilation (75 times/hr) on a 14-hr light/10-hr dark cycle at the Kobe University Life-Science Laboratory with
ad libitum access to water and a pellet diet (DC-8, Clea Japan, Tokyo,
Japan). This study was approved by the Institutional Animal Care and Use Committee
(Permission #25-06-03) and carried out according to the Kobe University Animal
Experimental Regulations.
