Individual lipid species were annotated according to sum composition of carbons and double bonds in the format Lipid Subclass (total number of carbons: total number of double bonds). If distinct fatty acid chains could be identified, they were annotated separated by an underscore (ex. PC 32:1, or PC (16:0_16:1). Using this approach, we cannot determine the sn-1 or sn-2 positions of the fatty acid chains. Lipid species within the sphingolipid category contain prefixes ‘d’ or ‘t’ to denote di-hydroxy or tri-hydroxy bases. For example, SM(d18:1_23:0) contains 2 hydroxyl groups. The Hex1Cer subclass is comprised of both glucosylceramide (GlcCer) and galactosylceramide (GalCer); the orientation of one of the hydroxyl groups in Glc differs from in Gal, and thus cannot be resolved by these methods 67 (link). Plasmanyl lipid species (ether linked) are annotated by ‘e’ and plasmenyl/plasmalogen (vinyl ether linked) lipid species are annotated by ‘p’ (ex. PC (36:5e) or PE (16:0p_20:4) 68 (link).
Comprehensive Lipid Profiling Protocol
Individual lipid species were annotated according to sum composition of carbons and double bonds in the format Lipid Subclass (total number of carbons: total number of double bonds). If distinct fatty acid chains could be identified, they were annotated separated by an underscore (ex. PC 32:1, or PC (16:0_16:1). Using this approach, we cannot determine the sn-1 or sn-2 positions of the fatty acid chains. Lipid species within the sphingolipid category contain prefixes ‘d’ or ‘t’ to denote di-hydroxy or tri-hydroxy bases. For example, SM(d18:1_23:0) contains 2 hydroxyl groups. The Hex1Cer subclass is comprised of both glucosylceramide (GlcCer) and galactosylceramide (GalCer); the orientation of one of the hydroxyl groups in Glc differs from in Gal, and thus cannot be resolved by these methods 67 (link). Plasmanyl lipid species (ether linked) are annotated by ‘e’ and plasmenyl/plasmalogen (vinyl ether linked) lipid species are annotated by ‘p’ (ex. PC (36:5e) or PE (16:0p_20:4) 68 (link).
Corresponding Organization : Massachusetts General Hospital
Other organizations : CHDI Foundation
Variable analysis
- None explicitly mentioned
- Glycerophospholipids
- Glycerolipids
- Sphingolipids
- Sterol lipids
- Fatty acyls
- Prenol lipids
- None explicitly mentioned
- No positive or negative controls were specified by the authors.
Annotations
Based on most similar protocols
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
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