RNAase free conditions (tools, solutions, handling) were followed throughout the experiment. The experiment was performed as per previously described protocol [52 (link)]. In brief, banana callus (24W) containing embryos were fixed at -23°C under vacuum using tissue freezing medium (Leica biosystems, Germany). Tissue blocks were fixed with the holding clamp of cryomicrotome (Leica biosystems, Germany) and 10–12 μm thin sections were prepared. These sections were taken on slides, air-dried at room temperature and observed under LCM microscope (Zeiss, Germany).
For microdissection, embryos were identified and marked using PALM (Zeiss, Germany) tool. Tissues were snipped-off with a laser beam along with marking in RNase-free tubes and stored at -80°C for RNA isolation.
For microdissection, embryos were identified and marked using PALM (Zeiss, Germany) tool. Tissues were snipped-off with a laser beam along with marking in RNase-free tubes and stored at -80°C for RNA isolation.
Free full text: Click here
