Thermostability and Protease Sensitivity of Antifungal Metabolites from Bacillus siamensis

The thermostability of the antifungal metabolites produced by the endophyte Bacillus siamensis CNE6 was studied by keeping the CFS in a boiling water bath for 10 min. In addition, to check the proteinaceous nature of the antifungal principles, CFS was treated with proteinase K (1 mg/mL) (HiMedia, India) for 2 h at 37°C (48 (link)). The antifungal potential of both heat-killed and proteinase K-treated CFS was studied against the isolated pathogen CRP1 by using the agar well diffusion method (44 (link)). Untreated CFS was used as the control.

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Gorai P.S., Ghosh R., Ghosh S., Samanta S., Sen A., Panja S., Gond S.K, & Mandal N.C. (2023). Management of Black Root Disease-Causing Fungus Fusarium solani CRP1 by Endophytic Bacillus siamensis CNE6 through Its Metabolites and Activation of Plant Defense Genes. Microbiology Spectrum, 11(2), e03082-22.

Publication 2023

proteinase K

Agar Antifungal Bacillus siamensis Bath Diffusion Endophyte Pathogen Proteinaceous Proteinase k

Corresponding Organization : Visva-Bharati University

Other organizations : Bankura Sammilani Medical College, Bose Institute, Ministry of AYUSH, Banaras Hindu University

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Variable analysis

independent variables

Heat treatment: Keeping the cell-free supernatant (CFS) in a boiling water bath for 10 minutes
Proteinase K treatment: Treating the CFS with proteinase K (1 mg/mL) for 2 hours at 37°C

dependent variables

Antifungal potential of the heat-killed and proteinase K-treated CFS against the isolated pathogen CRP1, measured using the agar well diffusion method

control variables

Untreated CFS, used as the control

controls

Positive control: Untreated CFS
Negative control: Not mentioned

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