Murine Models of Hemostasis and Thrombosis

BALB/c, C57BL/6, and splenectomized BALB/c mice were purchased from Charles River Canada. IL-4Rα/GPIbα-tg [91 (link)], GPIb^−/− [92 (link)], β3^−/− [93 (link)], and Asgr2^−/− [94 (link)] mice were gifts from J. Ware, Z. M. Ruggeri, R. O. Hynes, and K. Hoffmeister, respectively. FVIII-deficient (FVIII^null) mice were a gift from H. Kazazian. 2bF8^Tg mice, which are transgenic animals with platelet-specific human B domain-deleted FVIII expression, were generated by lentiviral transgenesis as previously reported [60 (link),95 (link)]. All mice were used between 8 and 16 weeks for experiments. All animal studies were approved by the Institutional Animal Care and Use Committee of either the St. Michael’s Hospital, Canada, or the Medical College of Wisconsin, USA.

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Li J., Karakas D., Xue F., Chen Y., Zhu G., Yucel Y.H., MacParland S.A., Zhang H., Semple J.W., Freedman J., Shi Q, & Ni H. (2023). Desialylated Platelet Clearance in the Liver is a Novel Mechanism of Systemic Immunosuppression. Research, 6, 0236.

Publication 2023

BALB/c C57BL/6

Animal Balb c mice Gifts Gpibα Human Institutional animal care and use committee Mice Platelet River Transgenic animals

Corresponding Organization :

Other organizations : University of Toronto, St. Michael's Hospital, Canadian Blood Services, Versiti Blood Center of Wisconsin, Medical College of Wisconsin, Toronto Metropolitan University, Toronto General Hospital, CARE Canada, Children's Hospital of Wisconsin, Children’s Institute

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Variable analysis

independent variables

Mouse strains: BALB/c, C57BL/6, splenectomized BALB/c, IL-4Rα/GPIbα-tg, GPIb^{-/-}, β3^{-/-}, Asgr2^{-/-}, FVIII-deficient (FVIII^null), and 2bF8^Tg

dependent variables

Measured outcomes (not explicitly mentioned)

control variables

All mice were used between 8 and 16 weeks for experiments
Animal studies were approved by the Institutional Animal Care and Use Committee of either the St. Michael's Hospital, Canada, or the Medical College of Wisconsin, USA

controls

Positive controls: Not explicitly mentioned
Negative controls: Not explicitly mentioned

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