Genotyping of PICALM and APOE

Genomic DNA was isolated using the Wizard Genomic DNA purification kit (Promega, Madison, WI, USA) from 4-ml peripheral blood samples according to the manufacturer's protocol. Genotyping of PICALM rs541458 and APOE was performed using the iPLEX Assay (SEQUENOM iPLEX® Gold Reagent Kit) according to the assay instructions for PCR amplification, SAP treatment, adjusting extension primers, iPLEX reaction, resin extraction, addition to SpectroCHIP bioarray, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis. Details were described previously (24 (link)).

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Zhuang L., Liu X., Shi Y., Liu X, & Luo B. (2019). Genetic Variants of PICALM rs541458 Modulate Brain Spontaneous Activity in Older Adults With Amnestic Mild Cognitive Impairment. Frontiers in Neurology, 10, 494.

Publication 2019

Wizard Genomic DNA purification kit iPLEX® Gold Reagent Kit

Apoe Assay Blood Genomic Gold Iplex Matrix assisted laser desorption ionization mass spectrometry Primers Promega Resin

Corresponding Organization :

Other organizations : Zhejiang Hospital, Zhejiang University, Zhongda Hospital Southeast University

Top 5 similar protocols

Variable analysis

independent variables

PICALM rs541458 genotyping
APOE genotyping

dependent variables

Peripheral blood samples

control variables

Use of Wizard Genomic DNA purification kit (Promega) for DNA isolation
Use of iPLEX Assay (SEQUENOM iPLEX® Gold Reagent Kit) for genotyping
Details of PCR amplification, SAP treatment, adjusting extension primers, iPLEX reaction, resin extraction, addition to SpectroCHIP bioarray, and matrix-assisted laser desorption ionization time-of-flight mass spectrometry analysis

controls

No positive or negative controls were explicitly mentioned in the provided information.

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